基于美洲蜚蠊下顎須的整合受體生物傳感器測(cè)定抗壞血酸

報(bào)道了以美洲蜚蠊的下顎須作為分子識(shí)別和轉(zhuǎn)換元件研制的一種新型生物組織傳感器,發(fā)現(xiàn)該傳感器對(duì)抗壞血酸(AA) 有選擇性的響應(yīng),響應(yīng)脈沖的發(fā)放頻率與AA 的濃度對(duì)數(shù)成正比,在5 ×10 - 5至5 ×10 - 2 mol/ L 范圍內(nèi)呈線性關(guān)系,檢測(cè)下限低于10 - 6 mol/ L AA. 該傳感器響應(yīng)快,并具有較好的重現(xiàn)性.
關(guān)鍵詞　生物傳感器,化學(xué)受體,美洲蜚蠊,下顎須
Abstract 　An intact2chemoreceptor2based biosensor based on the gustatory organ , the maxillary palp of Periplaneta americana , is reported , in which the maxillary palp serves as not only the molecular recognition elements but also the biological transducers. The binding events between ligand and chemoreceptor induce action potentials (APs) whose frequency correlates directly to the concentration of stimulants. The APs were recorded with a three2electrode system and collected by sound card method developed by our group. This is the first time that the biosensor is reported to yield quantitative linear dose2response data over the concentration range from 5 ×10 - 5 to 5 ×10 - 2 mol/ L. The new biosensor possesses some advantages over the biosensor based on isolated receptor : shorter response time ( < 1 s) , higher degrees of sensitivity (detect2limitation below 10 - 6 mol/ L) , broader dynamic response range (over four orders of magnitude) and more preferable reproducibility.
Keywords 　chemoreceptor , biosensor , Periplaneta americana , maxillary palp